Purpose
This kit allows for the determination of TAT concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human TAT level in the sample，use Purified Human TAT antibody to coat microtiter plate wells, make solid-phase antibody, then add TAT to wells, Combined TAT antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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