The recombinant mouse IL-21 protein is expressed in E. coli, whose amino acid sequence is derived from soluble form of mouse IL21 (Q9ES17), (Pro 25-Ser 146) was expressed, with an initial Met at the C-terminus.
The recombinant mouse IL21 consists of 123 amino acids and has a calculated molecular mass of 14.3KDa. It migrates as a 15 kDa band in SDS-PAGE under reducing conditions.
Mouse IL-21 protein measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 4-20 ng/ml.
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