The deduced 855-amino acid protein has a predicted molecular mass of 93 kD and contains 9 potential N-glycosylation sites. PRSS36 contains a signal peptide, followed by a propeptide domain, an active serine protease domain with the catalytic his, asp, and ser residues, and 2 serine protease domains predicted to be catalytically inactive. The first serine protease domain shares amino acid identity of 40 to 42% with gamma-1 tryptase (TPSG1), pancreasin, matriptase (ST14;), matriptase-2 (TMPRSS6), and polyserase-1 (TMPRSS9). Northern blot analysis detected a 5-kb transcript in fetal kidney and adult skeletal muscle, liver, placenta, and heart. A 2.2-kb transcript was detected in placenta, prostate, and colon and lung adenocarcinoma cell lines, and may be produced by alternative splicing.
Human Polyserase-2 (PRSS36) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS36 in samples. An antibody specific for PRSS36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS36 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS36 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS36 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Polyserase-2 (PRSS36) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-polyserase-2-prss36-elisa-kit-kte61103/
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