Enteropeptidase is an enzyme involved in human digestion. It is produced by cells in the duodenum wall, and is secreted from duodenum's glands, the crypts of Lieberkühn, whenever ingested food enters the duodenum from the stomach. Enteropeptidase has the critical job of turning trypsinogen (a zymogen) to trypsin, indirectly activating a number of pancreatic digestive enzymes.Enteropeptidase is a serine protease enzyme (EC 3.4.21.9) which converts inactive trypsinogen into active trypsin by cleavage at the C-terminal end of the sequence. It consists of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which is the catalytic subunit.
Human Enteropeptidase (PRSS7) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS7 in samples. An antibody specific for PRSS7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Enteropeptidase (PRSS7) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-enteropeptidase-prss7-elisa-kit-kte61102/
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