WNT signaling controls many fundamental processes during animal development. WNT transduction is mediated by the association of beta-catenin with nuclear TCF DNA-binding factors.Lgs encodes the homolog of human BCL9, and the authors provided genetic and molecular evidence that these proteins exert their function by physically linking Pygo to beta-catenin. The PYGO1 and PYGO2 proteins, which contain 419 and 406 amino acids, respectively, possess a highly conserved PHD finger that interacts with homology domain-1 (HD1) of BCL9. The findings suggested that the recruitment of PYGO permits beta-catenin to transcriptionally activate WNT target genes and raised the possibility that a deregulation of these events may play a causal role in the development of B-cell malignancies.
Human Pygopus homolog 1 (PYGO1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYGO1 in samples. An antibody specific for PYGO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYGO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYGO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYGO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Pygopus homolog 1 (PYGO1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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