Human Ras-related protein Rab-10 (RAB10) ELISA Kit,Abbkine_specification/price/image

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RAB10 belongs to the RAS superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking. The deduced 49-amino acid sequence includes the first 2 GTP-binding regions and effector domain residues that are conserved in RAB proteins. The deduced 200-amino acid RAB10 protein contains 4 highly conserved GTPase motifs and a C-terminal geranylgeranylation motif. Northern blot analysis detected a major transcript of about 4 kb and a smaller minor transcript. Highest expression was detected in heart and skeletal muscle, followed by brain, placenta, lung, liver, kidney, pancreas, testis, and spleen. Low expression was detected in ovary, prostate, and colon, and little to no expression was detected in thymus, small intestine, and leukocytes.

Human Ras-related protein Rab-10 (RAB10) ELISA Kit employs a two-site sandwich ELISA to quantitate RAB10 in samples. An antibody specific for RAB10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Ras-related protein Rab-10 (RAB10) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.