Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit,Abbkine_specification/price/image

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RAD51 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51 in samples. An antibody specific for RAD51 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAD51 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD51 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD51 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-dna-repair-protein-rad51-homolog-1-rad51-elisa-kit-kte60984/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.