Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) ELISA Kit,Abbkine_specification/price/image

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RAF proto-oncogene serine/threonine-protein kinase (RAF1) is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in RAF1 are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) ELISA Kit employs a two-site sandwich ELISA to quantitate RAF1 in samples. An antibody specific for RAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.