Anterior gradient homolog 2 (AGR2) is a human protein originally discovered in Xenopus laevis, encoded for by the gene of the same name. In Xenopus AGR2 plays a role in cement gland differentiation, however in human cancer cell lines high levels of AGR2 correlate with downregulation of the p53 response and AGR2 levels are elevated in the preneoplastic tissue Barrett's oesophagus.The deduced 175-amino acid soluble AGR2 protein, which is 91% identical to the mouse protein and 47% identical to the frog protein, contains a signal peptide. Northern blot analysis revealed strongest expression of 0.9- and 1.6-kb AGR2 transcripts in lung and in all ER-positive breast carcinoma lines tested; weaker expression was detected in pancreas.
Human Anterior gradient protein 2 homolog (AGR2) ELISA Kit employs a two-site sandwich ELISA to quantitate AGR2 in samples. An antibody specific for AGR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Anterior gradient protein 2 homolog (AGR2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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