In humans, Agmatinase is encoded by the AGMAT gene. In enzymology, an agmatinase is an enzyme that catalyzes the chemical reaction This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is agmatine amidinohydrolase. Other names in common use include agmatine ureohydrolase, and SpeB. This enzyme participates in urea cycle and metabolism of amino groups.
Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit employs a two-site sandwich ELISA to quantitate AGMAT in samples. An antibody specific for AGMAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGMAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGMAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGMAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-agmatine-ureohydrolase-agmatinase-agmat-elisa-kit-kte60964/
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