The sequence of the complete open reading frame for this fusion transcript revealed that the MLL protein is homologous with DNA methyltransferase, the Drosophila trithorax gene product, and the 'AT-hook' motif of high mobility group proteins. An alternative splice that deletes the AT-hook region of MLL was identified. AF4 is a serine- and proline-rich putative transcription factor with a glutamine-rich carboxyl terminus.The composition of the complete MLL-AF4 fusion product argues that it may act through either a gain-of-function or a dominant-negative mechanism in leukemogenesis. This gene is also symbolized MLLT2
Human AF4/FMR2 family member 1 (AFF1) ELISA Kit employs a two-site sandwich ELISA to quantitate AFF1 in samples. An antibody specific for AFF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human AF4/FMR2 family member 1 (AFF1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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