ADAM8 is expressed in macrophages, granulocytes, monocytes and B cells. It is an osteoclast stimulating factor. ADAM8 may play a role in neuron-glia interactions during neurodegeneration. It may also mediate extravasation of leukocytes either by degrading the vascular basement membrane or by liberating active molecules from their precursors harbored in the cell surface of leukocytes and endothelial cells.Human ADAM8 precursor consists of 824 amino acid (aa) residues consisting of a 16 aa signal peptide, a 637 aa ectodomain including pro-, metalloprotease, disintegrin-like and cysteine-rich regions, a 25 aa transmembrane domain, and a 146 aa cytoplasmic domain). ADAM8 naturally occurs as both a membrane and a soluble form.
Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM8 in samples. An antibody specific for ADAM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-a-disintegrin-and-metalloprotease-8-adam8-elisa-kit-kte60916/
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