Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids.ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.
Human Aminoacylase-1 (ACY1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACY1 in samples. An antibody specific for ACY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Aminoacylase-1 (ACY1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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