Beta-actin (gene name ACTB) is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.In a neoplastic cell line resulting from treatment of cultured human diploid fibroblasts with a chemical mutagen, Leavitt et al. (1982) observed a mutant form of beta actin. Toyama and Toyama (1984) isolated and characterized lines of KB cells resistant to cytochalasin B. They found that one resistant line had an alteration in beta-actin. Such cells bound less cytochalasin B than did parental KB cells. The authors suggested that the primary site of action of cytochalasin B on cell motility processes is beta-actin.
Human β-actin ELISA Kit employs a two-site sandwich ELISA to quantitate ACTB in samples. An antibody specific for ACTB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human β-actin ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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