Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit,Abbkine_specification/price/image

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Fatty acids are incorporated into membranes and signaling molecules and have roles in energy storage and metabolism. These essential functions require activation of the fatty acid by acyl-coenzyme A (CoA) synthetases, such as ACSF2, which form an activating thioester linkage between the fatty acid and CoA.The deduced 615-amino acid protein contains all 5 motifs characteristic of acyl-CoA synthetases. Phylogenetic analysis revealed that ACSF2 belongs to a small group of acyl-CoA synthetases that differ significantly from all other clades of acyl-CoA synthetases and from each other. FLJ20920 contains a PPARG-recognition element in its promoter sequence. Transfection of antisense FLJ20920 into preadipocytes resulted in decreased triglyceride accumulation and reduced expression of GLUT4 (SLC2A4) and HSL (LIPE) mRNA.

Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSF2 in samples. An antibody specific for ACSF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACSF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACSF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Acyl-CoA synthetase family member 2, mitochondrial (ACSF2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.