Prostatic acid phosphatase (PAP) is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases.The highest levels of acid phosphatase are found in metastasized prostate cancer. Diseases of the bone, such as Paget's disease or hyperparathyroidism, diseases of blood cells, such as sickle-cell disease or multiple myeloma or lysosomal storage diseases, such as Gaucher's disease, will show moderately increased levels.Certain medications can cause temporary increases or decreases in acid phosphatase levels. Manipulation of the prostate gland through massage, biopsy or rectal exam before a test may increase the level.Its physiological function may be associated with the liquefaction process of semen.
Human Prostatic acid phosphatase (ACPP) ELISA Kit employs a two-site sandwich ELISA to quantitate ACPP in samples. An antibody specific for ACPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACPP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Prostatic acid phosphatase (ACPP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-prostatic-acid-phosphatase-acpp-elisa-kit-kte60883/
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