Lysosomal acid phosphatase is composed of two subunits, alpha and beta, and is chemically and genetically distinct from red cell acid phosphatase. Lysosomal acid phosphatase 2 is a member of a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate.ACP2 is the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Human Lysosomal acid phosphatase (ACP2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACP2 in samples. An antibody specific for ACP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Lysosomal acid phosphatase (ACP2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-lysosomal-acid-phosphatase-acp2-elisa-kit-kte60881/
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