ATP citrate lyase is an enzyme that represents an important step in fatty acid biosynthesis.ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated in by insulin.The enzyme is composed of two subunits in green plants, species of fungi, Glaucophytes, Chlamydomonas, and prokaryotes.Animal ACL enzymes are homomeric, presumably an evolutionary fusion of the ACLA and ACLB genes probably occurred early in the evolutionary history of this kingdom.
Human ATP-citrate synthase (ACLY) ELISA Kit employs a two-site sandwich ELISA to quantitate ACLY in samples. An antibody specific for ACLY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACLY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACLY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACLY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human ATP-citrate synthase (ACLY) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-atp-citrate-synthase-acly-elisa-kit-kte60879/
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