Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit,Abbkine_specification/price/image

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ACAT2 is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.As the human TCP1 gene had been assigned to 6q25-q27 by study of somatic cell hybrids and by in situ hybridization, the ACAT2 gene was suspected to be localized to the same chromosome region. Retention of this close linkage during mammalian evolution suggests the possibility of some functional significance. Transcription of both DNA strands at a given locus is common in prokaryotic and viral systems. For examples of overlapping transcriptional units in humans.

Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACAT2 in samples. An antibody specific for ACAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-acetyl-coa-acetyltransferase-cytosolic-acat2-elisa-kit-kte60874/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.