Human Multidrug resistance-associated protein 4 (ABCC4) ELISA Kit,Abbkine_specification/price/image

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ABCC4, also known as MRP4, belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus.The insertion was attributed to 2 additional exons, designated 1a and 1b, that produced premature termination codons (PTCs). A comparison of human, monkey, and rodent ABCC4 genes revealed that these same PTC-producing exons were also highly conserved in evolution. In vitro inhibition of protein synthesis (using puromycin or anisomycin) selectively reduced nonsense-mediated mRNA decay of PTC-containing ABCC4 transcripts in all cell lines tested.

Human Multidrug resistance-associated protein 4 (ABCC4) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC4 in samples. An antibody specific for ABCC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Multidrug resistance-associated protein 4 (ABCC4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-multidrug-resistance-associated-protein-4-abcc4-elisa-kit-kte60861/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.