Human Multidrug resistance-associated protein 7 (ABCC10) ELISA Kit,Abbkine_specification/price/image

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Members of the ABC family of membrane proteins, such as ABCC10, are involved in energy-dependent transport of a wide variety of substrates across membranes. The 1,492-amino acid ABCC10 protein has a calculated molecular mass of 162 kD. It contains 2 nucleotide-binding folds, 17 predicted transmembrane segments arranged in 3 transmembrane domains, and 3 predicted N-glycosylation sites. Northern blot analysis was unable to detected ABCC10 expression, but RT-PCR detected ABCC10 expression in all 10 tissues examined. In vitro-translated ABCC10 had an apparent molecular mass of 158 kD by SDS-PAGE.Basal MRP7 promoter activity relied upon a proximal segment of the 5-prime flanking region bearing an E2F site acting cooperatively with 2 closely positioned SP1 sites.

Human Multidrug resistance-associated protein 7 (ABCC10) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC10 in samples. An antibody specific for ABCC10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Multidrug resistance-associated protein 7 (ABCC10) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-multidrug-resistance-associated-protein-7-abcc10-elisa-kit-kte60855/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.