Human 8-Hydroxy-desoxyguanosine (8-OHdG) ELISA Kit,Abbkine_specification/price/image

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8-oxo-7,8-dihydro-2 deoxyguanosine(8-OHdG), is probably the most important product of "oxidative stress” in DNA. Its concentration in DNA is, in fact. a quantitative analysis of the degree of DNA damage that an organism has undergone. Due to the importance of 8-OHdG of nucleic acidg in mutagenesis, carcinogenesis and aging, numerous chemical and biological investigations have been made on this subject in the past time. Kuchino and co-workers have found that 8-OHdG residue in DNA is misreading during the process of DNA replication. Recently, some reports have been presented on high 8-OHdG levels in patients suffering from various diseases such as chronic hepatitis, Fanconi s anemia, diabetes mellitus and Helicobacter pylori infections. As a result, 8-OHdG is a useful marker for the study of DNA damage arising from reactive oxygen species and is of great significance for cancer research.

Human 8-Hydroxy-desoxyguanosine (8-OHdG) ELISA Kit employs a two-site sandwich ELISA to quantitate 8-OHdG in samples. An antibody specific for 8-OHdG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any8-OHdG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 8-OHdG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 8-OHdG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human 8-Hydroxy-desoxyguanosine (8-OHdG) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.