6-keto-Prostaglandin F1a is the physiologically active and stable metabolite of prostacyclin. (A prostaglandin found in nearly all mammalian tissue that is a powerful vasodilator and inhibits platelet aggregation; it is biosynthesized enzymatically from prostaglandin endoperoxides in human vascular tissue; the sodium salt has been also used to treat primary pulmonary hypertension (Hypertension, Pulmonary).
Human 6-keto-prostaglandin F1a (6-K-PGF1a) ELISA Kit employs a two-site sandwich ELISA to quantitate 6ketoPGF1a in samples. An antibody specific for 6ketoPGF1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any6ketoPGF1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 6ketoPGF1a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 6ketoPGF1a bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 6-keto-prostaglandin F1a (6-K-PGF1a) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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