RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene.Retinol-binding protein-4 (RBP4) is the carrier protein involved in the transport of retinol from the liver storage site to peripheral tissue. On the other hand, cellular RBP (CRBP) is the intracellular carrier involved in intracellular movement of retinol.Colantuoni et al. (1985) isolated and sequenced a cDNA clone for the human CRBP gene.
Human Retinol-binding protein 1 (RBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP1 in samples. An antibody specific for RBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Retinol-binding protein 1 (RBP1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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