Regucalcinis a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in two transcript variants having different 5' UTRs, but encoding the same protein.Regucalcin is a proposed name for a calcium-binding protein that was discovered in 1978.Regucalcin differs from calmodulin and other Ca2+-related proteins as it does not contain an EF-hand motif of Ca2+-binding domain. It may regulate the effect of Ca2+ on liver cell functions.
Human Regucalcin (RGN) ELISA Kit employs a two-site sandwich ELISA to quantitate RGN in samples. An antibody specific for RGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Regucalcin (RGN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-regucalcin-rgn-elisa-kit-kte60802/
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