Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

RIOK1 includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. RioK1 and pICln bind to PRMT5 in a mutually exclusive fashion. This results in a PRMT5-WD45/MEP50 core structure that either associates with pICln or RioK1 in distinct complexes. RioK1 functions in analogy to pICln as an adapter protein by recruiting the RNA-binding protein nucleolin to the PRMT5 complex for its symmetrical methylation. The exclusive interaction of PRMT5 with either pICln or RioK1 thus provides the first mechanistic insight into how a methyltransferase can distinguish between its substrate proteins.

Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate RIOK1 in samples. An antibody specific for RIOK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRIOK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIOK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIOK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-serine-threonine-protein-kinase-rio1-riok1-elisa-kit-kte60795/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.