Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit,Abbkine_specification/price/image

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Eosinophil-derived neurotoxin is a distinct cationic protein of the eosinophil's large specific granule known primarily for its ability to induce ataxia, paralysis, and central nervous system cellular degeneration in experimental animals (Gordon phenomenon). Rosenberg et al. (1989) isolated a 725-bp cDNA clone for EDN. The open reading frame encodes a 134-amino acid polypeptide with a molecular mass of 15.5 kD and a 27-residue N-terminal hydrophobic leader sequence. The sequence of the mature polypeptide was identical to that reported for human urinary ribonuclease and to the N-terminal sequence of human liver ribonuclease. Similarities to the ribonucleases of pancreas and angiogenin indicate that EDN belongs to the ribonuclease multigene family. This gene is also symbolized RNS2 for ribonuclease 2.

Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE2 in samples. An antibody specific for RNASE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRNASE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.