SCGN contains 6 tandem repeats of the EF-hand Ca(2+)-binding domain, with a loop at all 6 putative Ca(2+)-binding sites, and shares significant similarity with calbindin and calretinin. Much lower expression of only the smaller transcript was detected in adrenal medulla, stomach, thyroid, colon, and small intestine.Immunohistochemical analysis showed SCGN expression restricted to the islet of Langerhans in pancreas, in C cells near thyroid follicles, and at sites typically occupied by neuroendocrine cells in all other tissues examined. Cell fractionation and confocal imaging of transfected rat insulinoma cells detected Scgn predominantly in the cytosolic fraction, with much less Scgn in nuclei. SCGN was expressed in a reticular pattern in transfected COS cells.
Human Secretagogin (SCGN) ELISA Kit employs a two-site sandwich ELISA to quantitate SCGN in samples. An antibody specific for SCGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCGN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCGN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Secretagogin (SCGN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-secretagogin-scgn-elisa-kit-kte60738/
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