Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit,Abbkine_specification/price/image

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By real-time PCR, Schulze-Tanzil et al. (2004) found that expression of SCX, a tendon-specific marker, increased in human tenocytes during their entire time in high-density cultures.By in situ hybridization, Brent et al. (2003) found that Scx was expressed in axial and ventrolateral body wall tendons during chicken embryonic development. It was also expressed in a population of tendon progenitors in developing somites.Brent et al. (2003) determined that FGF signaling in the myotome was both necessary and sufficient for induction of Scx expression in developing chicken somites. Overexpression of Pax1 inhibited Scx expression in somites.Hartz (2004) mapped the SCX gene to chromosome 8q24.3 based on an alignment of the SCX sequence with the genomic sequence.

Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit employs a two-site sandwich ELISA to quantitate SCXA in samples. An antibody specific for SCXA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCXA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCXA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCXA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.