SEMG2 is involved in the formation of a gel matrix that encases ejaculated spermatozoa. Proteolysis by the prostate-specific antigen (PSA) breaks down the gel matrix and allows the spermatozoa to move more freely. The encoded protein is found in lesser abundance than a similar semenogelin protein. The genes encoding these two semenogelin proteins are found in a cluster on chromosome 20.The transcription units are composed of 3 exons, of which the first encodes the signal peptide, the second encodes secreted protein, and the third contains solely 3-prime, noncoding nucleotides. The nucleotide sequences exhibited a similarity of close to 90% in the exons and greater than 80% in the introns and flanking nucleotides.
Human Semenogelin-2 (SEMG2) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMG2 in samples. An antibody specific for SEMG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Semenogelin-2 (SEMG2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-semenogelin-2-semg2-elisa-kit-kte60709/
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