Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit,Abbkine_specification/price/image

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Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42,000 Mr with serpin-like functional properties. Remold-O'Donnell et al. (1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6 kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity. The cDNA open reading frame encoded a 379-amino acid protein. Its sequence established EI as a member of the serpin superfamily. Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin. Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI).

Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB1 in samples. An antibody specific for SERPINB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.