Maspin is a serpin and tumor suppressor gene.It has been associated with prostate cancer and breast cancer.It has also been associated with tumors of the pancreas.It was identified in 1994.In normal cells expressing SERPINB5, the SERPINB5 promoter is unmethylated and the promoter region has acetylated histones and an accessible chromatin structure. In contrast, normal cells that do not express SERPINB5 have a completely methylated SERPINB5 promoter with hypoacetylated histones, an inaccessible chromatin structure, and a transcriptional repression that is relieved by inhibition of DNA methylation. These findings indicated that cytosine methylation is important in the establishment and maintenance of cell type-restricted gene expression.
Human Serpin B5 (SERPINB5) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB5 in samples. An antibody specific for SERPINB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Serpin B5 (SERPINB5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-serpin-b5-serpinb5-elisa-kit-kte60698/
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