Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit,Abbkine_specification/price/image

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By searching an EST database for sequences resembling CD33 (SIGLEC3) and screening a spleen cDNA library, Munday et al. (2001) isolated a cDNA encoding SIGLEC10. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and 4 C2-set Ig-like domains, 5 potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8.

Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit employs a two-site sandwich ELISA to quantitate SIGLEC10 in samples. An antibody specific for SIGLEC10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIGLEC10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Sialic acid-binding Ig-like lectin 10 (SIGLEC10) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.