S-phase kinase-associated protein 1 is a member of the SCF ubiquitin ligase protein complex. It binds to proteins containing an F-box motif, such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis.The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
Human S-phase kinase-associated protein 1 (SKP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP1 in samples. An antibody specific for SKP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human S-phase kinase-associated protein 1 (SKP1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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