Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit,Abbkine_specification/price/image

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Sodium/bile acid cotransporters are integral membrane glycoproteins. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization (SLC10A2;), and the other being found in the basolateral membranes of hepatocytes (SLC10A1). SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Alanine insertion experiments confirmed that 7 of the 9 hydrophobic stretches are membrane-integrated, with secondary structures and transport activity sensitive to positional displacement. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane.

Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC10A1 in samples. An antibody specific for SLC10A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC10A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC10A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC10A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-sodium-bile-acid-cotransporter-slc10a1-elisa-kit-kte60644/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.