AIM1 (absent in melanoma-1) is a novel gene whose expression is altered in association with tumor suppression in a model of human melanoma . Chromosome banding analysis of human malignant melanoma documented nonrandom alteration in chromosome 6.All known members of this superfamily contain 2 or 4 characteristic motifs arranged in 1 or 2 symmetrical domains. AIM1, in contrast, contains 12 beta-gamma motifs, suggesting a 6-domain structure resembling a trimer of beta- or gamma-crystallin subunits. The structure of the AIM1 gene shows remarkable similarity to the beta-crystallin genes, with homologous introns delineating equivalent protein structural units.
Human Absent in melanoma 1 protein (AIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate AIM1 in samples. An antibody specific for AIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Absent in melanoma 1 protein (AIM1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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