This transporter, SLC15A2, belongs to the same gene family as SLC15A1, the proton-coupled peptide transporter found in the small intestine.The SLC15A2 cDNA encodes a predicted 729-amino acid protein that contains 12 transmembrane domains and a long hydrophilic segment between transmembrane domains 9 and 10.The amino acid sequence of SLC15A2 shows 50% identity and 70% similarity with that of SLC15A1. Functional expression of the SLC15A2 cDNA in HeLa cells resulted in the induction of a proton-dependent transport system that was able to transport dipeptides, tripeptides, and beta-lactam antibiotics; free amino acids were not accepted as substrates. RT-PCR showed that SLC15A2 is expressed in kidney but not in intestine.
Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A2 in samples. An antibody specific for SLC15A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC15A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC15A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC15A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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