Human Vesicular acetylcholine transporter (SLC18A3) ELISA Kit,Abbkine_specification/price/image

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SLC18A3 transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. the human vesicular acetylcholine transporter is encoded by a sequence contained uninterrupted within the first intron of the gene for choline acetyltransferase, the enzyme required for acetylcholine biosynthesis in the peripheral and central cholinergic nervous systems. Thus, the VACHT gene is located at 10q11.2. Transcription of VACHT and CHAT mRNA from the same or contiguous promoters within the single regulatory locus provides a previously undescribed genetic mechanism for coordinate regulation of 2 proteins whose expression is required to establish a mammalian neuronal phenotype.

Human Vesicular acetylcholine transporter (SLC18A3) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC18A3 in samples. An antibody specific for SLC18A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC18A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC18A3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC18A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Vesicular acetylcholine transporter (SLC18A3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-vesicular-acetylcholine-transporter-slc18a3-elisa-kit-kte60621/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.