Human Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit,Abbkine_specification/price/image

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The major reabsorptive mechanism for D-glucose in the kidney is known to involve a lower affinity, high capacity Na(+)/glucose cotransporter, which is located in the early proximal convoluted tubule segment S1 and has a Na(+)-to-glucose coupling ratio of 1:1.Uptake experiments using cRNA-injected Xenopus oocytes showed specific uptake of glucose and alpha-methyl glucopyranoside at up to three times background. This uptake was saturable, suggesting that the cDNA corresponds to a low-affinity kidney sodium-glucose transporter. It was therefore referred to as sodium-glucose cotransporter 2, or SGLT2. SGLT2 mediates saturable Na(+)-dependent and phlorizin-sensitive transport with K(m) values consistent with low affinity Na(+)/glucose cotransport.

Human Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC5A2 in samples. An antibody specific for SLC5A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC5A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC5A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC5A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.