Human Sodium- and chloride-dependent creatine transporter 1 (SLC6A8) ELISA Kit,Abbkine_specification/price/image

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The creatine-phosphocreatine shuttle has important functions in the temporal and spatial maintenance of the energy supply to skeletal and cardiac muscle. Muscle cells do not synthesize creatine, but take it up via a special sodium-dependent transporter, the creatine transporter (SLC6A8). In mouse skin, they found high amounts of cytosolic brain CK (CKB) coexpressed with lower amounts of ubiquitous mitochondrial CK (CKMT1B), both mainly localized in suprabasal layers of the dermis, different cell types of hair follicles, sebaceous glands, and the subcutaneous panniculus carnosus muscle. Except for sebaceous glands, these cells also expressed CRT. Ckb and Crt were upregulated about 3-fold immediately after wounding of mouse skin, whereas the amount of Ckmt1b increased 10 to 15 days after wounding.

Human Sodium- and chloride-dependent creatine transporter 1 (SLC6A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A8 in samples. An antibody specific for SLC6A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Sodium- and chloride-dependent creatine transporter 1 (SLC6A8) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.