Human B (0,+)-type amino acid transporter 1 (SLC7A9) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

The SLC7A9 cDNA is polyadenylated and contains an open reading frame encoding a 487-amino acid protein. The protein, designated b(0,+)AT for b(0,+) amino acid transporter, belongs to a family of light subunits of amino acid transporters expressed in kidney, liver, small intestine, and placenta. Northern blot analysis revealed that the SLC7A9 gene was expressed as an approximately 1.9-kb transcript in these tissues. The tissue distribution of b(0,+)AT was consistent with that of a renal basic amino acid transporter (see SLC3A1, or rBAT) light subunit. As expected, b(0,+)AT brought rBAT to the plasma membrane in cotransfected COS cells. In contrast, transfection of rBAT alone resulted in the blockage of the expressed protein in the endoplasmic reticulum.

Human B (0,+)-type amino acid transporter 1 (SLC7A9) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC7A9 in samples. An antibody specific for SLC7A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human B (0,+)-type amino acid transporter 1 (SLC7A9) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-b-0-type-amino-acid-transporter-1-slc7a9-elisa-kit-kte60585/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.