The antiporter is a ubiquitous membrane-bound enzyme involved in volume- and pH-regulation of vertebrate cells. It is inhibited by the non-specific diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others.The gene was first disrupted in mouse fibroblasts. The lost function was then restored by transfection with human genomic DNA. Clones containing these specific human sequences were isolated. One genomic fragment was identified as an exon-coding sequence from the sodium-hydrogen ion antiporter gene by demonstration that it could complement antiporter deficiency in mouse cells; that it recognized an mRNA in cells expressing antiport activity but not in deficient cells; and that it was amplified in variants overexpressing antiport activity.
Human Sodium/hydrogen exchanger 1 (SLC9A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC9A1 in samples. An antibody specific for SLC9A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC9A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC9A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC9A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Sodium/hydrogen exchanger 1 (SLC9A1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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