Slit2 plays a vital role in axon guidance by signaling through Robo receptors. Recent evidence suggests that Slit2 protein may function in other settings because human and Xenopus Slit2 has been shown to inhibit leukocyte chemotaxis. SLIT2 protein is a putative ligand for the ROBO receptors. ROBO1 is inactivated by promoter region hypermethylation in <20% of human cancers. Slit2 is an excellent candidate tumor suppressor gene for colorectal cancer，and that it epigenetically silenced in >40% of lung and breast tumors. SLIT2 promoter region methylation was found in 23 (72%) of 32 primary colorectal cancers. In contrast, normal colorectal mucosa from the same patients exhibited significantly lower levels of SLIT2 promoter region hypermethylation.
Human Slit homolog 2 protein (SLIT2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT2 in samples. An antibody specific for SLIT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Slit homolog 2 protein (SLIT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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