The collagen cross-links were significantly diminished in Sh clones, and, for the major cross-link, dihydroxylysinonorleucine (DHLNL), glucosylgalactosyl-DHLNL was diminished with a concomitant increase in galactosyl-DHLNL. When subjected to in vitro incubation, in Sh clones, the rate of decrease in DHLNL was lower, whereas the rate of increase in its maturational cross-link, pyridinoline, was comparable with controls.
Human Dihydroxylysinonorleucine (DHLNL) ELISA Kit employs a two-site sandwich ELISA to quantitate DHLNL in samples. An antibody specific for DHLNL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHLNL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHLNL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHLNL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dihydroxylysinonorleucine (DHLNL) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-dihydroxylysinonorleucine-dhlnl-elisa-kit-kte62732/
bio-equip.cn
中文
