MEST encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. Mesoderm-specific transcript homolog protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for MEST. Pseudogenes of MEST are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.
Human Mesoderm-specific transcript homolog protein (MEST) ELISA Kit employs a two-site sandwich ELISA to quantitate MEST in samples. An antibody specific for MEST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Mesoderm-specific transcript homolog protein (MEST) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-mesoderm-specific-transcript-homolog-protein-mest-elisa-kit-kte61645/
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