Melanotransferrin encoded by MFI2 is a cell-surface glycoprotein found on melanoma cells. Melanotransferrin shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. MFI2 resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.
Human Melanotransferrin (MFI2) ELISA Kit employs a two-site sandwich ELISA to quantitate MFI2 in samples. An antibody specific for MFI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMFI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFI2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Melanotransferrin (MFI2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-melanotransferrin-mfi2-elisa-kit-kte61638/
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