Vinexin (SORBS3) encodes an SH3 domain-containing adaptor protein. The presence of SH3 domains play a role in this protein's ability to bind other cytoplasmic molecules and contribute to cystoskeletal organization, cell adhesion and migration, signaling, and gene expression. Multiple transcript variants encoding different isoforms have been found for SORBS3.
Human Vinexin (SORBS3) ELISA Kit employs a two-site sandwich ELISA to quantitate SORBS3 in samples. An antibody specific for SORBS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySORBS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORBS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORBS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Vinexin (SORBS3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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