SPATA5 encodes a member of the ATPase associated with diverse activities family, whose members are defined by a highly conserved ATPase domain. Members of this family participate in diverse cellular processes that include membrane fusion, DNA replication, microtubule severing, and protein degradation. The protein encoded by this gene has a putative mitochondrial targeting sequence and has been proposed to function in maintenance of mitochondrial function and integrity during mouse spermatogenesis. Allelic variants in SPATA5 have been associated with epilepsy, hearing loss, and mental retardation syndrome. Alternative splicing results in multiple transcript variants.
Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SPATA5 in samples. An antibody specific for SPATA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPATA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPATA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPATA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Spermatogenesis-associated protein 5 (SPATA5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-spermatogenesis-associated-protein-5-spata5-elisa-kit-kte60546/
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