Spartin (SPG20) encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome).
Human Spartin (SPG20) ELISA Kit employs a two-site sandwich ELISA to quantitate SPG20 in samples. An antibody specific for SPG20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPG20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPG20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPG20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Spartin (SPG20) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-spartin-spg20-elisa-kit-kte60534/
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