Spindlin-1 (SPIN1) is a chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed:24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes. May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.
Human Spindlin-1 (SPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPIN1 in samples. An antibody specific for SPIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Spindlin-1 (SPIN1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-spindlin-1-spin1-elisa-kit-kte60524/
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